Genetic variations on 31 and 450 residues of influenza A nucleoprotein affect viral replication and translation
Su-Jhen Hung, Yin-Mei Hsu, Sheng-Wen Huang, Huey-Pin Tsai, Leo Yi Yang Lee, Aeron C Hurt, Ian G Barr, Shin-Ru Shih, Jen-Ren Wang
JOURNAL OF BIOMEDICAL SCIENCE | BMC | Published : 2020
BACKGROUND: Influenza A viruses cause epidemics/severe pandemics that pose a great global health threat. Among eight viral RNA segments, the multiple functions of nucleoprotein (NP) play important roles in viral replication and transcription. METHODS: To understand how NP contributes to the virus evolution, we analyzed the NP gene of H3N2 viruses in Taiwan and 14,220 NP sequences collected from Influenza Research Database. The identified genetic variations were further analyzed by mini-genome assay, virus growth assay, viral RNA and protein expression as well as ferret model to analyze their impacts on viral replication properties. RESULTS: The NP genetic analysis by Taiwan and global sequen..View full abstract
Awarded by National Health Research Institutes
Awarded by Ministry of Science and Technology
This study was financially supported by grants from the National Health Research Institutes (grant no. IV-107-PP-13 and IV-107-SP-11); Ministry of Science and Technology (grant no. MOST 102-2321-B-006-023 and 104-2321-B-006-002); Centers of Disease Control, Ministry of Health and Welfare; and Center of Infectious Diseases and Signaling Research, National Cheng Kung University, Aim for the Top University Project, Ministry of Education, Taiwan.