Journal article

Genetic variations on 31 and 450 residues of influenza A nucleoprotein affect viral replication and translation

Su-Jhen Hung, Yin-Mei Hsu, Sheng-Wen Huang, Huey-Pin Tsai, Leo Yi Yang Lee, Aeron C Hurt, Ian G Barr, Shin-Ru Shih, Jen-Ren Wang

JOURNAL OF BIOMEDICAL SCIENCE | BMC | Published : 2020

Abstract

BACKGROUND: Influenza A viruses cause epidemics/severe pandemics that pose a great global health threat. Among eight viral RNA segments, the multiple functions of nucleoprotein (NP) play important roles in viral replication and transcription. METHODS: To understand how NP contributes to the virus evolution, we analyzed the NP gene of H3N2 viruses in Taiwan and 14,220 NP sequences collected from Influenza Research Database. The identified genetic variations were further analyzed by mini-genome assay, virus growth assay, viral RNA and protein expression as well as ferret model to analyze their impacts on viral replication properties. RESULTS: The NP genetic analysis by Taiwan and global sequen..

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