Comparative Transcriptomic and Functional Assessments o Linezolid-Responsive Small RNA Genes in Staphylococcus aureus
Wei Gao, Romain Guerillot, Ya Hsun Lin, Jai Tree, Marie Beaume, Patrice Francois, Ian R Monk, Torsten Seemann, Jacques Schrenzel, Benjamin P Howden, Timothy P Stinear
MSYSTEMS | AMER SOC MICROBIOLOGY | Published : 2020
Staphylococcus aureus contains a repertoire of at least 50 and possibly 500 small RNAs (sRNAs). The functions of most sRNAs are not understood, although some are known to respond to environmental changes, including the presence of antibiotics. Here, in an effort to better understand the roles of sRNAs in the context of antibiotic exposure, we took a clinical methicillin-resistant S. aureus (MRSA) isolate and separately deleted eight sRNAs that were significantly upregulated in response to the last-line antibiotic linezolid as revealed by transcriptome sequencing (RNA-seq) comparisons. We also deleted an additional 10 sRNAs that were either highly expressed or previously found to respond to a..View full abstract
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Awarded by National Medical Research Council of Australia
This project was supported by the National Medical Research Council of Australia (GNT1105525 [T.P.S.] and GNT1105905 and GNT1026656 [B.P.H.]).