Comparative Transcriptomic and Functional Assessments of Linezolid-Responsive Small RNA Genes in Staphylococcus aureus

W Gao; R Guérillot; YH Lin; J Tree; M Beaume; P François; IR Monk; T Seemann; J Schrenzel; BP Howden; TP Stinear

Journal article

Comparative Transcriptomic and Functional Assessments of Linezolid-Responsive Small RNA Genes in Staphylococcus aureus

W Gao, R Guérillot, YH Lin, J Tree, M Beaume, P François, IR Monk, T Seemann, J Schrenzel, BP Howden, TP Stinear

Msystems | AMER SOC MICROBIOLOGY | Published : 2020

DOI: 10.1128/mSystems.00665-19

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Abstract

Staphylococcus aureus contains a repertoire of at least 50 and possibly 500 small RNAs (sRNAs). The functions of most sRNAs are not understood, although some are known to respond to environmental changes, including the presence of antibiotics. Here, in an effort to better understand the roles of sRNAs in the context of antibiotic exposure, we took a clinical methicillin-resistant S. aureus (MRSA) isolate and separately deleted eight sRNAs that were significantly upregulated in response to the last-line antibiotic linezolid as revealed by transcriptome sequencing (RNA-seq) comparisons. We also deleted an additional 10 sRNAs that were either highly expressed or previously found to respond to a..

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University of Melbourne Researchers

Romain Guérillot Author

Ian Monk Author

Torsten Seemann Author

Ben Howden Author

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Grants

Awarded by National Health and Medical Research Council

Funding Acknowledgements

This project was supported by the National Medical Research Council of Australia (GNT1105525 [T.P.S.] and GNT1105905 and GNT1026656 [B.P.H.]).The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.