Journal article

Analysis of Global Collection of Group A Streptococcus Genomes Reveals that the Majority Encode a Trio of M and M-Like Proteins

Hannah R Frost, Mark R Davies, Valerie Delforge, Dalila Lakhloufi, Martina Sanderson-Smith, Velusamy Srinivasan, Andrew C Steer, Mark J Walker, Bernard Beall, Anne Botteaux, Pierre R Smeesters

mSphere | AMER SOC MICROBIOLOGY | Published : 2020

Abstract

The core Mga (multiple gene activator) regulon of group A Streptococcus (GAS) contains genes encoding proteins involved in adhesion and immune evasion. While all GAS genomes contain genes for Mga and C5a peptidase, the intervening genes encoding M and M-like proteins vary between strains. The genetic make-up of the Mga regulon of GAS was characterized by utilizing a collection of 1,688 GAS genomes that are representative of the global GAS population. Sequence variations were examined with multiple alignments, and the expression of all core Mga regulon genes was examined by quantitative reverse transcription-PCR in a representative strain collection. In 85.2% of the sampled genomes, the Mga l..

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Grants

Awarded by Belgian Fonds National de la Recherche Scientifique


Funding Acknowledgements

H.R.F. is supported by a Ph.D. fellowship granted by the Royal Academy of Medicine from Belgium and sponsored by GSK Vaccines. The work is supported by the Belgian Fonds National de la Recherche Scientifique research grants (PDR T.0255.16 and CDR J.0019.17). The funders had no role in study design and analysis.