Journal article

Combined optogenetic and electrical stimulation of auditory neurons increases effective stimulation frequency-an in vitro study

William L Hart, Rachael T Richardson, Tatiana Kameneva, Alex C Thompson, Andrew K Wise, James B Fallon, Paul R Stoddart, Karina Needham



OBJECTIVE: The performance of neuroprostheses, including cochlear and retinal implants, is currently constrained by the spatial resolution of electrical stimulation. Optogenetics has improved the spatial control of neurons in vivo but lacks the fast-temporal dynamics required for auditory and retinal signalling. The objective of this study is to demonstrate that combining optical and electrical stimulation in vitro could address some of the limitations associated with each of the stimulus modes when used independently. APPROACH: The response of murine auditory neurons expressing ChR2-H134 to combined optical and electrical stimulation was characterised using whole cell patch clamp electrophy..

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Awarded by Action on Hearing Loss International project

Awarded by ARC Training Centre in Biodevices

Funding Acknowledgements

This work is supported by Action on Hearing Loss International project grant G89 and the ARC Training Centre in Biodevices (IC140100023). William Hart is supported by an Australian Government Research Training Program (RTP) Scholarship. The COP4*H134R/EYFP mouse line was generously provided by Professor Heather Young of the University of Melbourne, Australia and the Cre-parvalbumin mice were kindly provided by Prof Steven Petrou of the Florey Institute of Neuroscience, Australia. The Bionics Institute acknowledges the support it receives from the Victorian Government through its Operational Infrastructure Support Program. WH, RR, AT, AW, JF, PS, KN disclose a potential financial conflict of interest related to a patent application.