Journal article

Clinical application of embryo aneuploidy testing by next-generation sequencing.

Carmen Rubio, Lorena Rodrigo, Carmen Garcia-Pascual, Vanessa Peinado, Inmaculada Campos-Galindo, Sandra Garcia-Herrero, Carlos Simón

Biol Reprod | Published : 2019

Abstract

We review here the evolution in the field of embryo aneuploidy testing over the last 20 years, from the analysis of a subset of chromosomes by fluorescence in situ hybridisation to the transition toward a more comprehensive analysis of all 24 chromosomes. This current comprehensive aneuploidy testing most commonly employs next-generation sequencing (NGS). We present our experience in over 130 000 embryo biopsies using this technology. The incidence of aneuploidy was lower in trophectoderm biopsies compared to cleavage-stage biopsies. We also confirmed by NGS that embryo aneuploidy rates increased with increasing maternal age, mostly attributable to an increase in complex aneuploid embryos. I..

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University of Melbourne Researchers