ROR gamma t( ) Treg to Th17 ratios correlate with susceptibility to Giardia infection
Ivet A Yordanova, Alba Cortes, Christian Klotz, Anja A Kuehl, Markus M saa Heimet, Cinzia Cantacessi, Susanne Hartmann, Sebastian Rausch
SCIENTIFIC REPORTS | NATURE PUBLISHING GROUP | Published : 2019
Infections with Giardia are among the most common causes of food and water-borne diarrheal disease worldwide. Here, we investigated Th17, Treg and IgA responses, and alterations in gut microbiota in two mouse lines with varying susceptibility to Giardia muris infection. Infected BALB/c mice shed significantly more cysts compared with C57BL/6 mice. Impaired control of infection in BALB/c mice was associated with lower Th17 activity and lower IgA levels compared with C57BL/6 mice. The limited metabolic activity, proliferation and cytokine production of Th17 cells in BALB/c mice was associated with higher proportions of intestinal Foxp3+RORγt+ regulatory T cells and BALB/c mice developed increa..View full abstract
Awarded by German Research Foundation (DFG)
Awarded by German Federal Ministries of Education and Research (BMBF)
The authors would like to thank Yvonne Weber, Marion Muller, Bettina Sonnenburg, Christiane Palissa and Beate Anders for providing excellent technical support, as well as to the GRK2046 student assistant Martin Lamatsch for his help with sample collection and processing. Gratitudes are also extended to Gernot Reifenberger from the Charite for his assistance with the qPCR-based fecal microbiota analysis. This study was supported by the German Research Foundation (DFG) GRK2046 Research Training Program (CK, SH and SR) and a 3-year scholarship from the German Academic Exchange Service (DAAD) awarded to IAY. MMH received support from the German Federal Ministries of Education and Research (BMBF) in frame of the zoonoses research consortium PAC-Campylobacter (IP7/01KI1725D). AC is supported by a postdoctoral fellowship from Fundacion Alfonso Martin Escudero (Madrid, Spain). Research in the CC laboratory is funded by grants from the Royal Society, the Horserase Betting Levy Board, the Biotechnology and Biological Sciences Research Council (BBSRC) and the Isaac Newton Trust.