Journal article

P. falciparum In Vitro Killing Rates Allow to Discriminate between Different Antimalarial Mode-of-Action

Laura M Sanz, Benigno Crespo, Cristina De-Cozar, Xavier C Ding, Jose L Llergo, Jeremy N Burrows, Jose F Garcia-Bustos, Francisco-Javier Gamo

PLoS ONE | PUBLIC LIBRARY SCIENCE | Published : 2012

Abstract

Chemotherapy is still the cornerstone for malaria control. Developing drugs against Plasmodium parasites and monitoring their efficacy requires methods to accurately determine the parasite killing rate in response to treatment. Commonly used techniques essentially measure metabolic activity as a proxy for parasite viability. However, these approaches are susceptible to artefacts, as viability and metabolism are two parameters that are coupled during the parasite life cycle but can be differentially affected in response to drug actions. Moreover, traditional techniques do not allow to measure the speed-of-action of compounds on parasite viability, which is an essential efficacy determinant. W..

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University of Melbourne Researchers