Mass Spectrometry Reveals New Insights into the Production of Superoxide Anions and 4-Hydroxynonenal Adducted Proteins in Human Sperm
Jacob Kendal Netherton, Louise Hetherington, Rachel Anne Ogle, Marum Mazloumi Gavgani, Tony Velkov, Ana Izabel Bilbin Villaverde, Nuch Tanphaichitr, Mark Andrew Baker
Proteomics | WILEY | Published : 2020
The free-radical theory of male infertility suggests that reactive oxygen species produced by the spermatozoa themselves are a leading cause of sperm dysfunction, including loss of sperm motility. However, the field is overshadowed on several fronts, primarily because: i) the probes used to measure reactive oxygen species (ROS) are imprecise; and ii) many reports suggesting that oxygen radicals are detrimental to sperm function add an exogenous source of ROS. Herein, a more reliable approach to measure superoxide anion production by human spermatozoa based on MS analysis is used. Furthermore, the formation of the lipid-peroxidation product 4-hydroxynonenal (4-HNE) during in vitro incubation ..View full abstract
The Authors thank the NHMRC and ARC for their support.