Circulating gluten-specific, but not CMV-specific, CD39( ) regulatory T cells have an oligoclonal TCR repertoire
Laura Cook, C Mee Ling Munier, Nabila Seddiki, Melinda Y Hardy, Robert P Anderson, John Zaunders, Jason A Tye-Din, Anthony D Kelleher, David van Bockel
Clinical and Translational Immunology | WILEY | Published : 2020
Objectives: Understanding the T cell receptor (TCR) repertoire of regulatory CD4+ T-cell (Treg) populations is important for strategies aiming to re-establish tolerance in autoimmune diseases. We studied circulating deamidated gluten-epitope-specific CD39+ Tregs in patients with coeliac disease following an oral gluten challenge, and we used cytomegalovirus (CMV)-specific CD39+ Tregs from healthy controls as a comparator population. Methods: We used the OX40 assay to isolate antigen-specific Tregs by induced surface co-expression of CD25, OX40 and CD39. RACE PCR amplification and Sanger sequencing of the TCR β chain were used to analyse repertoire diversity. Results: We found that, following..View full abstract
Awarded by NHMRC
This work was funded by the following sources: Australian Government Department of Health and Ageing; the NHMRC via a Program (510448) grant, NHMRC project grant (1085875), a Practitioner Fellowship (ADK); and a Coeliac Research Fund Grant (NS, RPA, JT-D and ADK), an Australian Postgraduate Award and a UNSW Research Excellence Scholarship (LC).