Journal article

Nicotinamide adenine dinucleotide induces a bivalent metabolism and maintains pluripotency in human embryonic stem cells

Jarmon G Lees, David K Gardner, Alexandra J Harvey

STEM CELLS | WILEY | Published : 2020

Abstract

Nicotinamide adenine dinucleotide (NAD+ ) and its precursor metabolites are emerging as important regulators of both cell metabolism and cell state. Interestingly, the role of NAD+ in human embryonic stem cell (hESC) metabolism and the regulation of pluripotent cell state is unresolved. Here we show that NAD+ simultaneously increases hESC mitochondrial oxidative metabolism and partially suppresses glycolysis and stimulates amino acid turnover, doubling the consumption of glutamine. Concurrent with this metabolic remodeling, NAD+ increases hESC pluripotent marker expression and proliferation, inhibits BMP4-induced differentiation and reduces global histone 3 lysine 27 trimethylation, plausibl..

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Grants

Awarded by Alfred Nicholas Fellowship Award


Awarded by Australian Research Council Special Research Initiative Stem Cells Australia


Awarded by Jasper Loftus-Hills Award


Funding Acknowledgements

Alfred Nicholas Fellowship Award, Grant/Award Number: UTR6.197; Australian Research Council Special Research Initiative Stem Cells Australia, Grant/Award Number: SR110001002; Jasper Loftus-Hills Award, Grant/Award Number: UTR7.116; Melbourne Research Fellowships (Career Interruption)