Tentacle Transcriptomes of the Speckled Anemone (Actiniaria: Actiniidae: Oulactis sp.): Venom-Related Components and Their Domain Structure
Michela L Mitchell, Gerry Q Tonkin-Hill, Rodrigo A Morales, Anthony W Purcell, Anthony T Papenfuss, Raymond S Norton
MARINE BIOTECHNOLOGY | SPRINGER | Published : 2020
Cnidarians are one of the oldest known animal lineages (ca. 700 million years), with a unique envenomation apparatus to deliver a potent mixture of peptides and proteins. Some peptide toxins from cnidarian venom have proven therapeutic potential. Here, we use a transcriptomic/proteomic strategy to identify sequences with similarity to known venom protein families in the tentacles of the endemic Australian 'speckled anemone' (Oulactis sp.). Illumina RNASeq data were assembled de novo. Annotated sequences in the library were verified by cross-referencing individuals' transcriptomes or protein expression evidence from LC-MS/MS data. Sequences include pore-forming toxins, phospholipases, peptida..View full abstract
Awarded by ARC
This project was funded in part by ARC linkage grant LP150100621. M. L.M. received an Australian Government Research Training Program Scholarship, Monash Medicinal Chemistry Faculty Scholarship and Monash University-Museum Victoria Scholarship top-up. R.S.N., A.T.P. and A.W.P. received fellowship support from the Australian National Health and Medical Research Council.