Journal article

Flexible polygon-mirror based laser scanning microscope platform for multiphoton in-vivo imaging

YX Li, V Gautam, A Brustle, IA Cockburn, VR Daria, C Gillespie, K Gaus, C Alt, WM Lee

JOURNAL OF BIOPHOTONICS | WILEY-V C H VERLAG GMBH | Published : 2017

Abstract

Commercial microscopy systems make use of tandem scanning i.e. either slow or fast scanning. We constructed, for the first time, an advanced control system capable of delivering a dynamic line scanning speed ranging from 2.7 kHz to 27 kHz and achieve variable frame rates from 5 Hz to 50 Hz (512 × 512). The dynamic scanning ability is digitally controlled by a new customized open-source software named PScan1.0. This permits manipulation of scanning rates either to gain higher fluorescence signal at slow frame rate without increasing laser power or increase frame rates to capture high speed events. By adjusting imaging speed from 40 Hz to 160 Hz, we capture a range of calcium waves and transie..

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University of Melbourne Researchers