Discrimination of Dental Pulp Stem Cell Regenerative Heterogeneity by Single-Cell Raman Spectroscopy
Amr Alraies, Elisabetta Canetta, Rachel J Waddington, Ryan Moseley, Alastair J Sloan
Tissue Engineering Part C: Methods | MARY ANN LIEBERT, INC | Published : 2019
IMPACT STATEMENT: This study is the first to investigate and confirm the effectiveness of single-cell Raman spectroscopy (SCRM), in its ability to discriminate between dental pulp stem cells (DPSCs) with contrasting proliferative and differentiation capabilities. The findings show that SCRM can rapidly and noninvasively distinguish and identify DPSC subpopulations in vitro with superior proliferative and multipotency properties, versus lesser quality DPSCs, thereby overcoming the significant heterogeneity issues surrounding DPSC ex vivo expansion and differentiation capabilities. Such findings support further SCRM assessment for the selective screening/isolation of superior quality DPSCs fro..View full abstract
This work was supported by PhD studentship funding, awarded to Dr. Amr Alraies by the Albawani Company, Saudi Arabia.