Journal article

Real-time binding kinetic analyses of the interaction of the dietary stain orange II with dentin matrix

Amr Alraies, David K Cole, Jeremy S Rees, Carl Glasse, Nigel Young, Rachel J Waddington, Alastair J Sloan

Journal of Dentistry | ELSEVIER SCI LTD | Published : 2019


OBJECTIVES: Dietary stains can be adsorbed into the dentin of teeth. Using Orange II as a model dietary stain, this study investigated the strength of its interaction with the mineral and protein components of dentin matrix and how hydrogen peroxide (H2O2) treatment influences this interaction. METHODS: Dentin slices were prepared from human teeth and were either deproteinized (5.6% sodium hypochlorite, 12 days), demineralised (0.5 M EDTA, 3 days) or left as intact control samples. Samples were stained with Orange II for 1-168 h, during which staining intensity was quantified by image analysis. Similarly, uptake of stain by deproteinized / demineralized samples treated with 10 or 30% H2O2 wa..

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University of Melbourne Researchers


Funding Acknowledgements

This research was entirely financed by Philips and did not receive any grants from external funding agencies. The authors declare no potential conflicts of interest with respect to the authorship and/or publication of this article.