ANALYSING THE BIOACTIVE MAKEUP OF DEMINERALISED DENTINE MATRIX ON BONE MARROW MESENCHYMAL STEM CELLS FOR ENHANCED BONE REPAIR
SJ Avery, L Sadaghiani, AJ Sloan, RJ Waddington
EUROPEAN CELLS & MATERIALS | AO RESEARCH INSTITUTE DAVOS-ARI | Published : 2017
Dentine matrix has proposed roles for directing mineralised tissue repair in dentine and bone; however, the range of bioactive components in dentine and specific biological effects on bone-derived mesenchymal stem cells (MSCs) in humans are less well understood. The aims of this study were to further elucidate the biological response of MSCs to demineralised dentine matrix (DDM) in enhancing wound repair responses and ascertain key contributing components. Dentine was obtained from human teeth and DDM proteins solubilised with ethylenediaminetetraacetic acid (EDTA). Bone marrow derived MSCs were commercially obtained. Cells with a more immature phenotype were then selected by preferential fi..View full abstract
Awarded by Rosetrees Trust, UK
Awarded by Medical Research Council
Awarded by Rosetrees Trust
The authors are grateful to the Rosetrees Trust, UK (Ref M309) for funding of this study.