Journal article

A Novel Ex vivo Culture Model for Inflammatory Bone Destruction

AJ Sloan, SY Taylor, EL Smith, JL Roberts, L Chen, XQ Wei, RJ Waddington

Journal of Dental Research | SAGE PUBLICATIONS INC | Published : 2013

Abstract

Pathological alterations in the balance of bone metabolism are central to the progression of inflammatory bone diseases such as periodontal disease. We have developed and characterized a novel ex vivo murine mandible model of inflammatory bone destruction. Slices of mandible were cultured for 14 days in the presence or absence of P. gingivalis lipopolysaccharide (LPS) or pro-inflammatory cytokines. Following culture, cell viability and tissue histomorphometry were assessed with quantification of matrix proteins, resident osteoclasts, ligament cells, monocytes, macrophages, and neutrophils. In the absence of inflammatory factors, culture viability, osteoclasts, and matrix components were main..

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University of Melbourne Researchers

Grants

Awarded by National Centre for the Replacement, Refinement and Reduction of Animals in Research (NC3Rs), UK


Awarded by National Natural Science Foundation of China


Awarded by Medical Research Council


Awarded by National Centre for the Replacement, Refinement and Reduction of Animals in Research (NC3Rs)


Funding Acknowledgements

This study was supported by the National Centre for the Replacement, Refinement and Reduction of Animals in Research (NC3Rs), UK (grant G0600349), and by the National Natural Science Foundation of China (funding 81070805). The authors declare no potential conflicts of interest with respect to the authorship and/or publication of this article.