Osteogenic Potential of Bone Marrow Stromal Cells on Smooth, Roughened, and Tricalcium Phosphate-Modified Titanium Alloy Surfaces
John S Colombo, Albert Carley, Garry JP Fleming, St John Crean, Alastair J Sloan, Rachel J Waddington
INTERNATIONAL JOURNAL OF ORAL & MAXILLOFACIAL IMPLANTS | QUINTESSENCE PUBLISHING CO INC | Published : 2012
PURPOSE: This study investigated the influence of smooth, roughened, and tricalcium phosphate (TCP)-coated roughened titanium-aluminum-vanadium (Ti-6Al-4V) surfaces on the osteogenic potential of rat bone marrow stromal cells (BMSCs). METHODS: Machined smooth (MS), grit-blasted roughened (MT), and roughened surfaces coated with TCP were prepared from Ti-6Al-4V. Plastic surfaces were used as a control. Surface topography and chemical characteristics were determined. Cell attachment, morphology, proliferation, and temporal expression of mRNA and protein markers associated with bone healing were examined. RESULTS: Roughness values were 0.09 ± 0.02 Μm, 2.71 ± 0.24 Μm, and 6.08 ± 0.62 Μm for MS, ..View full abstract
The authors wish to express their appreciation to General Implant Forum Training, the educational arm of Osteo-Ti, in providing financial support to John Colombo during his doctoral studies. In addition, the authors thank Dr Larry Fisher, National Institute of Dental and Craniofacial Research of the US National Institutes of Health, for the generous gift of polyclonal antibodies used in this study.