Long term effect of Low Intensity Pulsed Ultrasound on a human tooth slice organ culture

Abstract

Objective: Investigate the effect of therapeutic Low Intensity Pulsed Ultrasound (LIPUS) on human dentine-pulp complex in an in vitro model. Design: 92 premolars were extracted from 23 adolescent orthodontic patients. The premolars were sectioned transversely into 600 μm thick slices. The slices were divided into two main groups according to how often the LIPUS was applied (single or daily application), and then subdivided into five subgroups each (5, 10, 15 and 20 min and one control group). The tooth slices were cultured at (37 °C/5% CO 2) in a humidified incubator where medium was changed every 48 h. LIPUS was applied using a 3.9 cm 2 transducer that produces an incident intensity of 30 m..