Long term effect of Low Intensity Pulsed Ultrasound on a human tooth slice organ culture
Saleh Al-Daghreer, Michael Doschak, Alastair J Sloan, Paul W Major, Giseon Heo, Cristian Scurtescu, Ying Y Tsui, Tarek El-Bialy
Archives of Oral Biology | PERGAMON-ELSEVIER SCIENCE LTD | Published : 2012
OBJECTIVE: Investigate the effect of therapeutic Low Intensity Pulsed Ultrasound (LIPUS) on human dentine-pulp complex in an in vitro model. DESIGN: 92 premolars were extracted from 23 adolescent orthodontic patients. The premolars were sectioned transversely into 600 μm thick slices. The slices were divided into two main groups according to how often the LIPUS was applied (single or daily application), and then subdivided into five subgroups each (5, 10, 15 and 20 min and one control group). The tooth slices were cultured at (37 °C/5% CO(2)) in a humidified incubator where medium was changed every 48 h. LIPUS was applied using a 3.9 cm(2) transducer that produces an incident intensity of 30..View full abstract
Awarded by University of Alberta
Awarded by Medical Research Council
Awarded by National Centre for the Replacement, Refinement and Reduction of Animals in Research (NC3Rs)
Funding for this project was provided mainly by King Abdul Aziz University, Jeddah, Saudi Arabia and partially by the University of Alberta Fund for Dentistry, Grant 2007-03.