An ex vivo culture model for orthodontically induced root resorption

Abstract

Objectives: Root resorption is a ubiquitous although undesirable sequela to orthodontic treatment. Current methods to investigate the pathophysiology have certain limitations. In pursuit to understand and develop treatment modalities for orthodontically induced root resorption, the ability to manipulate cells within their natural extracellular matrix in a three dimensional organotypic model is invaluable. The study aimed to develop a laboratory-based organotypic model to investigate the effect of orthodontic forces on the periodontium. Methods: Mandibular slices of male Wistar rats were maintained in Trowel-typed cultures at 37 °C in 5% carbon dioxide in air for 7 days with test specimens su..