The effect of low intensity pulsed ultrasound in a 3D ex vivo orthodontic model

Abstract

Objectives: This study investigated the effects of low intensity pulsed ultrasound (LIPUS) on dentoalveolar structures during orthodontic force application using a novel organ culture system. Methods: Mandibles were dissected from 28-day-old male Sprague Dawley rats, sliced into 1.5 mm and cultured at 37 °C and 5% CO2, prior to application of a 50 g force to each mandible slice. Slices were randomly divided into three groups of control, 5 and 10 min LIPUS application and cultured for five days before histological and histomorphometrical analysis. Results: Cementum and predentine thickness and subodontoblast and periodontal ligament cell counts were increased in the ultrasound groups, with in..