Conference Proceedings

Bespoke human hypertrophic chondrocytic cell lines provide the osteoinductive signals required for vascularized bone formation

Bradley Stringer, Rachel Waddington, Alastair Sloan, Ian Phillips, Gary Telford, David Hughes, Geoffrey Craig, Lavinia Gangemi, Ian Brook, Christine Freeman, Xuesong Cao, Mandeep Gosal, Simon Smith, Graham Russell, George Foster

Tissue Engineering | MARY ANN LIEBERT, INC | Published : 2007


Hypertrophic cartilage provides the morphological and biochemical template for orchestrating bone growth. To produce a bone-inductive material such as hypertrophic cartilage for clinical use, we have conditionally immortalized hypertrophic chondrocytic cells from human femur and expanded them in vitro through more than 145 divisions. The clonal cell lines generated by this process consistently express signals that induce both rat and human marrow cells to differentiate in vitro into osteoblastic cells. Further, implantation of the cell-free extracellular matrix from the immortalized chondrocytic cells causes vascularized bone to form in vivo in bony defects, but not in ectopic sites such as ..

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University of Melbourne Researchers