Journal article

Phenotype and behaviour of dental pulp cells during expansion culture

Minal Patel, Anthony J Smith, Alastair J Sloan, Gay Smith, Paul R Cooper

Archives of Oral Biology | PERGAMON-ELSEVIER SCIENCE LTD | Published : 2009

Abstract

OBJECTIVE: Primary pulp cell cultures are frequently used to study cellular responses, odontogenic potential and stem cell responses. Their isolation and expansion via a range of technical approaches are widely reported. The purpose of this study was to investigate the influence of isolation approach and extended expansion on cell phenotype and behaviour. DESIGN: To determine viable cell isolation, enzymatic dissociation was performed on rodent incisor pulps using collagenase, trypsin, hyaluronidase and ficin. Extended expansion culture of released cells was performed in DMEM and alpha-MEM media. Cultures were subsequently analysed for gene expression, cell proliferation, cell morphology and..

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University of Melbourne Researchers