Phenotype and behaviour of dental pulp cells during expansion culture
Minal Patel, Anthony J Smith, Alastair J Sloan, Gay Smith, Paul R Cooper
Archives of Oral Biology | PERGAMON-ELSEVIER SCIENCE LTD | Published : 2009
OBJECTIVE: Primary pulp cell cultures are frequently used to study cellular responses, odontogenic potential and stem cell responses. Their isolation and expansion via a range of technical approaches are widely reported. The purpose of this study was to investigate the influence of isolation approach and extended expansion on cell phenotype and behaviour. DESIGN: To determine viable cell isolation, enzymatic dissociation was performed on rodent incisor pulps using collagenase, trypsin, hyaluronidase and ficin. Extended expansion culture of released cells was performed in DMEM and alpha-MEM media. Cultures were subsequently analysed for gene expression, cell proliferation, cell morphology and..View full abstract
We acknowledge funding of a PhD studentship to MP by the School of Dentistry, University of Birmingham, UK.Funding: School of Dentistry, University of Birmingham.