Technical Advances in the Sectioning of Dental Tissue and of On-Section Cross-Linked Collagen Detection in Mineralized Teeth
Sim K Singhrao, Alastair J Sloan, Emma L Smith, Charles W Archer
Microscopy Research and Technique | WILEY-LISS | Published : 2010
Immunohistochemical detection of cross-linked fibrillar collagens in mineralized tissues is much desired for exploring the mechanisms of biomineralization in health and disease. Mineralized teeth are impossible to section when embedded in conventional media, thus limiting on-section characterization of matrix proteins by immunohistochemistry. We hypothesized that by using an especially formulated acrylic resin suitable for mineralized dental tissues, not only sectioning of teeth would be possible, but also our recently developed immunofluorescence labeling technique would be amenable to fully calcified tissues for characterization of dentinal fibrillar collagens, which remains elusive. The h..View full abstract
Awarded by Technology Strategy Board
Awarded by National Centre for the Replacement, Refinement and Reduction of Animals in Research (NC3Rs)
Contract grant sponsor: Technology Strategy Board (NC3Rs project); Contract grant number: 77844.