Journal article

Parathyroid hormone receptor 1 (PTHR1) is a prognostic indicator in canine osteosarcoma

Awf A Al-Khan, Judith S Nimmo, Mourad Tayebi, Stewart D Ryan, James O Simcock, Raboola Tarzi, Charles A Kuntz, Eman S Saad, Michael J Day, Samantha J Richardson, Janine A Danks

SCIENTIFIC REPORTS | NATURE PUBLISHING GROUP | Published : 2020

Abstract

Osteosarcoma (OS) is the most common malignant primary bone tumour in humans and dogs. Several studies have established the vital role of parathyroid hormone-related protein (PTHrP) and its receptor (PTHR1) in bone formation and remodeling. In addition, these molecules play a role in the progression and metastasis of many human tumour types. This study investigated the expression of PTHR1 and PTHrP in canine OS tissues and assessed their prognostic value. Formalin-fixed, paraffin-embedded tissue samples from 50 dogs diagnosed with primary OS were immunolabeled with antibodies specific for PTHR1 and PTHrP. The immunostaining intensity of tumours from patients with OS was correlated with survi..

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Grants

Awarded by National Health and Medical Research Council


Funding Acknowledgements

This study was based on research carried out during a National Health and Medical Research Council Project Grant 620203. We acknowledge the scholarship support given by The Ministry of Higher Education in Oman, The Consulate General of the Sultanate of Oman in Melbourne to Awf Al-Khan and the School of Health and Biomedical Sciences at RMIT University for this research. The authors would like to thank Prof T. J. Martin for his thoughtful review and critique of the manuscript. We would like to thank the veterinary clinics in Melbourne that enthusiastically supported this research and provided survival time data. The authors thank Umma Habiba and Utpal Adhikari for performing immunofluorescence and western blotting.