Journal article

Breeding for boron tolerance in lentil (Lens culinaris Medik.) using a high-throughput phenotypic assay and molecular markers

Matthew S Rodda, Shimna Sudheesh, Muhammad Javid, Dianne Noy, Annathurai Gnanasambandam, Anthony T Slater, Garry M Rosewarne, Sukhjiwan Kaur

PLANT BREEDING | WILEY | Published : 2018

Abstract

This study describes the identification of a quantitative trait locus (QTL) in the recombinant inbred line population of ILL2024 × ILL6788 and subsequent validation of associated molecular markers. A high-quality genetic linkage map was constructed with 758 markers that cover 1,057 cM, with an average intermarker distance of 2 cM. QTL analysis revealed a single genomic region on Lc2 to be associated with B tolerance and accounted for up to 76% of phenotypic variation (Vp). The best markers for B tolerance were assessed for their utility in routine breeding applications using validation panels of diverse lentil germplasm and breeding material derived from ILL2024. A marker generated from the ..

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