Identification and mapping of Lr3 and a linked leaf rust resistance gene in durum wheat

Abstract

Leaf rust, caused by Puccinia triticina Eriks., is an important disease of durum wheat (Triticum turgidum ssp. durum) worldwide and can be controlled through the use of genetic resistance. Two leaf rust resistance genes in durum wheat lines 'Camayo' and 'Storlom' were mapped to chromosome 6BL via amplified fragment length polymorphism (AFLP) with bulked segregant analysis. The leaf rust resistance gene in Storlom was identified to be Lr3 using a previously known co-segregating marker. Xmwg798. We validated a sequence tagged site version of this marker and identified three AFLP markers that were associated with the resistance gene in Camayo. The lack of recombination between the two genes in ..