Journal article

Dynamic Structural Changes Accompany the Production of Dihydroxypropanesulfonate by Sulfolactaldehyde Reductase

Mahima Sharma, Palika Abayakoon, James P Lingford, Ruwan Epa, Alan John, Yi Jin, Ethan D Goddard-Borger, Gideon J Davies, Spencer J Williams



2,3-Dihydroxypropanesulfonate (DHPS) is a major sulfur species in the biosphere. One important route for the production of DHPS is sulfoglycolytic catabolism of sulfoquinovose (SQ) through the Embden-Meyerhof-Parnas (sulfo-EMP) pathway. SQ is a sulfonated carbohydrate present in plant and cyanobacterial sulfolipids (sulfoquinovosyl diacylglyceride and its metabolites) and is biosynthesized globally at a rate of around 10 billion tons per annum. The final step in the bacterial sulfo-EMP pathway involves reduction of sulfolactaldehyde (SLA) to DHPS, catalyzed by an NADH-dependent SLA reductase. Based on conserved sequence motifs, we assign SLA reductase to the β-hydroxyacid dehydrogenase (β-HA..

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Awarded by Australian Research Council

Awarded by Leverhulme Trust

Awarded by National Health and Medical Research Council

Funding Acknowledgements

We thank the Australian Research Council (DP180101957), the Leverhulme Trust (grant number RPG-2017-190), the National Health and Medical Research Council (GNT1139546 and GNT1139549), and the Royal Society for the Ken Murray Research Professorship to G.J.D. We thank Dr. Johan P. Turkenburg and Sam Hart for assistance with the X-ray data collection, and the Diamond Light Source for access to beamlines I04, i24, and I04-1 under proposal number mx-18598. We acknowledge Dr. Andrew Leech from Bioscience Technology Facility, University of York for assistance with the SEC-MALS analysis.