Journal article

Uptake and transcytosis of functionalized superparamagnetic iron oxide nanoparticles in an in vitro blood brain barrier model

Angela Ivask, Emily H Pilkington, Thomas Blin, Aleksandr Kakinen, Heiki Vija, Meeri Visnapuu, John F Quinn, Michael R Whittaker, Ruirui Qiao, Thomas P Davis, Pu Chun Ke, Nicolas H Voelcker

Biomaterials Science | ROYAL SOC CHEMISTRY | Published : 2018

Abstract

Two major hurdles in nanomedicine are the limited strategies for synthesizing stealth nanoparticles and the poor efficacy of the nanoparticles in translocating across the blood brain barrier (BBB). Here we examined the uptake and transcytosis of iron oxide nanoparticles (IONPs) grafted with biomimetic phosphorylcholine (PC) brushes in an in vitro BBB model system, and compared them with bare, PEG or PC-PEG mixture grafted IONPs. Hyperspectral imaging indicated IONP co-localization with cells. Quantitative analysis with total reflection X-ray fluorescence spectrometry showed that after 24 h, 78% of PC grafted, 68-69% of PEG or PC-PEG grafted, and 30% of bare IONPs were taken up by the BBB. Tr..

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Grants

Awarded by ARC Project


Awarded by Estonian Research Council


Awarded by European Regional Development Fund project


Funding Acknowledgements

The authors thank Ester Sporleder, Wing Yin Tong and Andrew Beck at the University of South Australia for assistance with DLS, endothelial cell and antibody staining and cryomicrotomy, Vivian Kuusk at the Estonian University of Life Sciences for assistance with ultramicrotome preparation. This work was partly supported by ARC Project No. CE140100036 (Davis, Voelcker), Future Industries Institute of the University of South Australia (Foundation Fellowship of Ivask), Estonian Research Council grants ETF9347, PUT748 and European Regional Development Fund project TK134 (Ivask, Vija). Davis is thankful for the award of an Australian Laureate Fellowship from the ARC.