Journal article

The Amount of BCL6 in B Cells Shortly after Antigen Engagement Determines Their Representation in Subsequent Germinal Centers

Marcus James Robinson, Zhoujie Ding, Catherine Pitt, Erica Janet Brodie, Isaak Quast, David Mathew Tarlinton, Dimitra Zotos

CELL REPORTS | CELL PRESS | Published : 2020

Abstract

It is unknown whether the incremental increases in BCL6 amounts in antigen-activated B cells influence the unfolding differentiation before germinal center (GC) formation. By comparing shortly after immunization the distribution of conventional B cells to those enforced to express BCL6 at the upper quartile of normal and those lacking BCL6 altogether, we determined that B cell representation in the stages before the GC compartment was related to BCL6 amounts. This was not by increased proliferation or suppression of early plasmablast differentiation, but rather by preferential recruitment and progression through these early stages of B cell activation, culminating in preferential transition ..

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University of Melbourne Researchers

Grants

Awarded by National Health and Medical Research Council (NHMRC) Australia


Awarded by Swiss National Science Foundation


Awarded by NHMRC Australia Peter Doherty Early Career fellowship


Awarded by Swedish International Postdoctoral Fellowship


Funding Acknowledgements

We thank Laura Pasqualucci and Ricardo Dalla-Favera for the ImBCL6 mice and the RIKEN BRC through the National Bio-Resource Project of the Ministry of Education, Culture, Sports, Science, and Technology (MEXT), Japan, for the B6.Cg-Tg(FucciS/G2/M)#492Bsi mice. We thank Pablo Fernandez De Canete Nieto and Carola Vinuesa (John Curtin School of Medicine, Canberra, Australia) for CD23 cre BCL6fl/fl tissues. We acknowledge the help of the Alfred Alliance Flow Cytometry Core Facility, Alfred Alliance Monash Intensive Care Unit, and Monash Animal Research Platform staff. The authors acknowledge the services of Micromon at Monash University. We thank Lars Nitschke for helpful discussions and Kristy O'Donnell for lab support. This work was funded by a National Health and Medical Research Council (NHMRC) Australia Program grant and research fellowship to D.M.T. (10549925 and 1060675), an Australian Postgraduate Award PhD Scholarship to D.Z., an Early Postdoc Mobility fellowship (P2ZHP3_164964) provided by the Swiss National Science Foundation and an NHMRC Australia Peter Doherty Early Career fellowship (APP1145136) to I.Q. Z.D. was supported by a Swedish International Postdoctoral Fellowship (2016-06659).