Journal article

Siglec-10 expression is up-regulated in activated human CD4( ) T cells

E Bandala-Sanchez, NG Bediaga, G Naselli, AM Neale, LC Harrison

HUMAN IMMUNOLOGY | ELSEVIER SCIENCE INC | Published : 2020

Abstract

Most sialic acid-binding immunoglobulin-like lectins (Siglecs) suppress immune cell function but are expressed at low levels on human T cells. We found that soluble CD52 inhibited T cell signalling by ligating Siglec-10, but the presence of Siglec-10 on human T cells has been questioned. To address this concern, we examined the expression of Siglec-10 at the RNA and protein level in human CD4+ T cells. Analysis by RNAseq, qPCR and flow cytometry demonstrated that, in contrast to other Siglecs, after activation of CD4+ T cells Siglec-10 was selectively upregulated in a subset of cells also high for CD52 expression. This observation is consistent with a homeostatic role for Siglec-10 in human ..

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Grants

Awarded by Australian National Health and Medical Research Council (NHMRC)


Awarded by NHMRC Senior Principal Research Fellowship


Funding Acknowledgements

This work was supported by an Australian National Health and Medical Research Council (NHMRC) Program Grant (10373210). LCH was the recipient of a NHMRC Senior Principal Research Fellowship (1080887).