Identification of bacteria in the Rocky Mountain wood tick, Dermacentor andersoni, using single-strand conformation polymorphism (SSCP) and DNA sequencing
Shaun J Dergousoff, Clare A Anstead, Neil B Chilton
EXPERIMENTAL AND APPLIED ACAROLOGY | SPRINGER | Published : 2020
PCR-based single-strand conformation polymorphism (SSCP) analyses combined with DNA sequencing of the prokaryotic 16S ribosomal (r) RNA gene encompassing the hypervariable V4 region was used to determine the bacterial composition of Rocky Mountain wood ticks (Dermacentor andersoni) attached to Richardson's ground squirrels (Urocitellus richardsonii) and questing on vegetation in southern Saskatchewan, Canada. The bacteria present in questing adult ticks from Saskatchewan Landing Provincial Park included Rickettsia peacockii, a Francisella-like endosymbiont (FLE) and an Arsenophonus-like endosymbiont. Bacteria in the adult and nymphal ticks attached to U. richardsonii collected from Beechy in..View full abstract
Funding for this work was provided from the Natural Sciences Research Council of Canada (NSERC) and the Canadian Foundation for Innovation Fund (to NBC). SJD received funding through a NSERC Postgraduate Scholarship. CAA received funding through the Margaret MacKay Scholarship and the University of Saskatchewan.