Journal article

Direct nanopore sequencing of mRNA reveals landscape of transcript isoforms in apicomplexan parasites

Vern Lee, Louise Judd, Aaron Jex, Kathryn Holt, Christopher Tonkin, Stuart Ralph

Cold Spring Harbor Laboratory | Published : 2020

Abstract

Alternative splicing is a widespread phenomenon in metazoans by which single genes are able to produce multiple isoforms of the gene product. However, this has been poorly characterised in apicomplexans, a major phylum of some of the most important global parasites. Efforts have been hampered by atypical transcriptomic features, such as the high AT content of Plasmodium RNA, but also the limitations of short read sequencing in deciphering complex splicing events. In this study, we utilised the long read direct RNA sequencing platform developed by Oxford Nanopore Technologies (ONT) to survey the alternative splicing landscape of Toxoplasma gondii and Plasmodium falciparum . We find that while..

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University of Melbourne Researchers

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