A global analysis of protein expression profiles in Sinorhizobium meliloti: discovery of new genes for nodule occupancy and stress adaptation.
Michael A Djordjevic, Han Cai Chen, Siria Natera, Giel Van Noorden, Christian Menzel, Scott Taylor, Clotilde Renard, Otto Geiger, Georg F Weiller, undefined Sinorhizobium DNA Sequencing Consortium
Molecular Plant-Microbe Interactions | Scientific Societies | Published : 2003
A proteomic examination of Sinorhizobium meliloti strain 1021 was undertaken using a combination of 2-D gel electrophoresis, peptide mass fingerprinting, and bioinformatics. Our goal was to identify (i) putative symbiosis- or nutrient-stress-specific proteins, (ii) the biochemical pathways active under different conditions, (iii) potential new genes, and (iv) the extent of posttranslational modifications of S. meliloti proteins. In total, we identified the protein products of 810 genes (13.1% of the genome's coding capacity). The 810 genes generated 1,180 gene products, with chromosomal genes accounting for 78% of the gene products identified (18.8% of the chromosome's coding capacity). The ..View full abstract