Journal article

Complementation of the Mycoplasma synoviae MS-H vaccine strain with wild-type oppF(1) influences its growth characteristics

Somayeh Kordafshari, Marc S Marenda, Rebecca Agnew, Pollob Shil, Muhammad A Shahid, Christina Marth, Barbara M Konsak, Amir H Noormohammadi

Avian Pathology | TAYLOR & FRANCIS LTD | Published : 2020

Abstract

The Mycoplasma synoviae (MS) vaccine strain MS-H harbours a frameshift mutation in oppF1 (oligopeptide permease transporter) which results in expression of a truncated OppF1. The effect of this mutation on growth and attenuation of the MS-H is unknown. In this study, the impact of the mutation on the vaccine phenotype was investigated in vitro by introducing a wild-type copy of oppF1 gene in the MS-H genome. Wild-type oppF1 was cloned under the vlhA promoter into an oriC vector carrying a tetracycline resistance gene. MS-H was successfully transformed with the final construct pMS-oppF1-tetM or with a similar vector lacking oppF1 coding sequence (pMS-tetM). The MS-H transformed with pMS-oppF1..

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