Construction of 3D native elemental maps for large biological specimens using LA-ICP-MS coupled with X-ray tomography

Abstract

A step-change in the utility of elemental imaging in the biological sciences can be achieved by the integration of quantitative elemental distributions with structural information, allowing novel insights into how tissue development is associated with a dynamic chemical environment. To demonstrate this potential, here we construct a 3D correlative map of biometal distribution and skeletal growth for the first time in a complex organism-in a mouse embryo at developmental stage E14. We achieved this by registering laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) data to micro-computerized tomography (μCT) imagery using endogenous fiducial markers. LA-ICP-MS compositional..