Journal article
Functional characterization and fitness cost of spinosad-resistant alleles in Ceratitis capitata
Ana Guillem-Amat, Enric Urena, Elena Lopez-Errasquin, Vicente Navarro-Llopis, Philip Batterham, Lucas Sanchez, Trent Perry, Pedro Hernandez-Crespo, Felix Ortego
JOURNAL OF PEST SCIENCE | SPRINGER HEIDELBERG | Published : 2020
Abstract
The sustainability of control programs for the Mediterranean fruit fly, Ceratitis capitata, for citrus crops in Spain has been threatened by the development of resistance to malathion and lambda-cyhalothrin in recent years. Spinosad is widely used without apparent loss of efficacy. However, a highly resistant strain, JW-100s, has been obtained after laboratory selection. Spinosad resistance in JW-100s has been associated with different mutant alleles of the α6 subunit of the nicotinic acetylcholine receptor (Ccα6) including an isoform-specific truncation allele, Ccα63aQ68*. Using the GAL4 > UAS system in Drosophila melanogaster to demonstrate expression of this truncated α6 subunit, in a dα6..
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Awarded by CICYT
Awarded by Spanish MINECO
Funding Acknowledgements
This work received financial support from CICYT (AGL2016-76516-R). The Spanish MINECO granted A. Guillem-Amat a predoc (BES-C-2014-068937) and a mobility (EEBB-I-16-11336) fellowships. We gratefully acknowledge Maria Torne (Dow Agro-Science Iberica) for providing technical grade spinosad, Charles Robin (University of Melbourne) for assisting with bureaucratic issues with the Australian Government, Tinna Yang (University of Melbourne) for the keeping and shipping of the flies and Sandra Vacas (Universitat Politecnica de Valencia) for the scientific advice on electroantennography.