Journal article

Cell-derived microvesicles in infective endocarditis: Role in diagnosis and potential for risk stratification at hospital admission

Milton Henriques Guimaraes Junior, Teresa Cristina Abreu Ferrari, Andrea Teixeira-Carvalho, Marcela de Lima Moreira, Lorena Jinia de Souza Santos, Matheus Fernandes Costa-Silva, Rodrigo Matos Pinto Coelho, Pedro Henrique Oliveira Murta Pinto, Tijmen Hermen Ris, Jonathas Teixeira Salles, Livia Silva Araujo Passos, Maria Carmo Pereira Nunes

Journal of Infection | W B SAUNDERS CO LTD | Published : 2019

Abstract

OBJECTIVES: To characterize the plasmatic profile of cell-derived microvesicles (MVs) at diagnosis and during the treatment of patients with infective endocarditis (IE). METHODS: Blood samples from 57 patients with IE were obtained on 3 consecutive moments: upon admission (T0), at 2 weeks (T1), and at the end of treatment (T2), and were compared with 22 patients with other bacterial infections. MPs were measured by flow cytometry and labeled for specific cell markers of CD45 (leukocytes), CD66b (neutrophils), CD14 (monocytes), CD41a (platelets), CD51 (endothelial cells), CD3 (T lymphocyte) and CD235a (erythrocytes). RESULTS: MVs from platelets (pltMVs), leukocytes (leukMVs), neutrophils (neu..

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University of Melbourne Researchers