Mass cytometry reveals cellular fingerprint associated with IgE plus peanut tolerance and allergy in early life
Melanie R Neeland, Sandra Andorf, Monali Manohar, Diane Dunham, Shu-Chen Lyu, Thanh D Dang, Rachel L Peters, Kirsten P Perrett, Mimi LK Tang, Richard Saffery, Jennifer J Koplin, Kari C Nadeau
Nature Communications | NATURE PUBLISHING GROUP | Published : 2020
IgE-mediated peanut allergic is common, often serious, and usually lifelong. Not all individuals who produce peanut-specific IgE will react upon consumption of peanut and can eat the food without adverse reactions, known as sensitized tolerance. Here, we employ high-dimensional mass cytometry to define the circulating immune cell signatures associated with sensitized tolerance and clinical allergy to peanut in the first year of life. Key features of clinical peanut allergic are increased frequency of activated B cells (CD19hiHLADRhi), overproduction of TNFα and increased frequency of peanut-specific memory CD4 T cells. Infants with sensitized tolerance display reduced frequency but hyper-res..View full abstract
Awarded by NIH
We thank the children and parents who participated in the HealthNuts study. We also thank current and past staff for recruiting and maintaining the cohort, and processing the data and biospecimens. We thank Matthew Kirkey for assistance with CyTOF experiments. This work was supported by funding from the NIH (5R01AI140134, 2U19AI104209, and 5U01AI140498), EAT (End Allergies Together), FARE (Food Allergy Research & Education), the Sean N. Parker Center for Allergy and Asthma Research at Stanford University, and the Kim and Ping Li endowment. The HealthNuts study is supported by funding from the National Health and Medical Research Council of Australia (NHMRC), the Ilhan Food Allergy Foundation, AnaplyaxiStop, The Charles and Sylvia Viertel Medical Research Foundation, and the Victorian Government's Operational Infrastructure Support Program. M.R.N. is supported by a Melbourne Children's Lifecourse Postdoctoral Fellowship.