Journal article

Volumetric mapping of the functional neuroanatomy of the respiratory network in the perfused brainstem preparation of rats

Rishi R Dhingra, Thomas E Dick, Werner I Furuya, Roberto F Galan, Mathias Dutschmann

JOURNAL OF PHYSIOLOGY-LONDON | WILEY | Published : 2020

Abstract

KEY POINTS: The functional neuroanatomy of the mammalian respiratory network is far from being understood since experimental tools that measure neural activity across this brainstem-wide circuit are lacking. Here, we use silicon multi-electrode arrays to record respiratory local field potentials (rLFPs) from 196-364 electrode sites within 8-10 mm3 of brainstem tissue in single arterially perfused brainstem preparations with respect to the ongoing respiratory motor pattern of inspiration (I), post-inspiration (PI) and late-expiration (E2). rLFPs peaked specifically at the three respiratory phase transitions, E2-I, I-PI and PI-E2. We show, for the first time, that only the I-PI transition enga..

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