Journal article
Identification by deep sequencing and profiling of conserved and novel hickory microRNAs involved in the graft process
Xiaojiao Sima, Bo Jiang, Jia Fang, Yongqin He, Zhongxiang Fang, Saravana KM Kumar, Wei Ren, Lingling Qiu, Xiaomin Chen, Bingsong Zheng
PLANT BIOTECHNOLOGY REPORTS | SPRINGER | Published : 2015
Abstract
MicroRNAs (miRNAs) play a vital role in plant development and growth through negative regulation of post-transcriptional gene expression. Carya cathayensis (hickory) is an important species for dried nuts and oil in China, with high nutritional and economic value. The graft technique is an important strategy for hickory cultivation. To understand the role of miRNAs involved in the hickory graft process, we constructed three small ribonucleic acid (RNA) libraries from hickory rootstock (2 years old) and scion (1 year old) at 0, 7, and 14 days post-graft. Sequence analysis of the three libraries identified 21 conserved miRNAs belonging to 13 families, and 10 novel and 8 potentially novel miRNA..
View full abstractGrants
Awarded by National Natural Science Foundation of China
Awarded by Zhejiang Provincial Natural Science Foundation for Distinguished Young Scholar
Awarded by Chinese Ministry of Education
Awarded by Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry
Awarded by National High Technology Research and Development Program of China (863 Program)
Awarded by initial project of Major State Basic Research Program of China
Awarded by Fruit Innovation Team Project of Zhejiang Province
Funding Acknowledgements
This study was supported by National Natural Science Foundation of China (31070604, 31270716 and 31470683); Zhejiang Provincial Natural Science Foundation for Distinguished Young Scholar (LR13C160001); Pre-research Foundation of Center for Cultivation of Subtropical Forest Resources (CCSFR), Zhejiang A & F University; Key Project of Chinese Ministry of Education (212072); Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry ([2009]1341); National High Technology Research and Development Program of China (863 Program) (2013AA102605); initial project of Major State Basic Research Program of China (2011CB111510); Fruit Innovation Team Project of Zhejiang Province (2009R50033). We would like to thank the reviewers for their helpful comments and proposals on the manuscript.