Journal article

Cobalt-Directed Assembly of Antibodies onto Metal-Phenolic Networks for Enhanced Particle Targeting.

Wenjie Zhang, Quinn A Besford, Andrew J Christofferson, Patrick Charchar, Joseph J Richardson, Aaron Elbourne, Kristian Kempe, Christoph E Hagemeyer, Matthew R Field, Chris F McConville, Irene Yarovsky, Frank Caruso

Nano Letters | ACS Publications | Published : 2020

Abstract

The orientation-specific immobilization of antibodies onto nanoparticles, to preserve antibody-antigen recognition, is a key challenge in developing targeted nanomedicines. Herein, we report the targeting ability of metal-phenolic network (MPN)-coated gold nanoparticles with surface-physisorbed antibodies against respective antigens. The MPN coatings were self-assembled from metal ions (FeIII, CoII, CuII, NiII, or ZnII) cross-linked with tannic acid. Upon physisorption of antibodies, all particle systems exhibited enhanced association with target antigens, with CoII systems demonstrating more than 2-fold greater association. These systems contained more metal atoms distributed in a way to sp..

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Grants

Awarded by Australian Research Council (ARC) Centre of Excellence in Convergent Bio-Nano Science and Technology


Awarded by ARC


Awarded by National Health and Medical Research Council (NHMRC)


Awarded by NHMRC


Funding Acknowledgements

This research was conducted and funded by the Australian Research Council (ARC) Centre of Excellence in Convergent Bio-Nano Science and Technology (project number CE140100036) and the ARC under the Discovery Project Scheme (DP170103331, F.C. and DP170100511, I.Y.). C.E.H. and F.C. acknowledge the award of a National Health and Medical Research Council (NHMRC) Research Fellowship (GNT1154270, C.E.H.) and an NHMRC Senior Principal Research Fellowship (GNT1135806, F.C.).