Journal article

Polyphenol-Mediated Assembly of Proteins for Engineering Functional Materials

Yiyuan Han, Zhixing Lin, Jiajing Zhou, Gyeongwon Yun, Rui Guo, Joseph J Richardson, Frank Caruso

Angewandte Chemie International Edition | John Wiley & Sons | Published : 2020

Abstract

Functional materials composed of proteins have attracted much interest owing to the inherent and diverse functionality of proteins. However, establishing general techniques for assembling proteins into nanomaterials is challenging owing to the complex physicochemical nature and potential denaturation of proteins. Here, a simple, versatile strategy is introduced to fabricate functional protein assemblies through the interfacial assembly of proteins and polyphenols (e.g., tannic acid) on various substrates (organic, inorganic, and biological). The dominant interactions (hydrogen-bonding, hydrophobic, and ionic) between the proteins and tannic acid were elucidated; most proteins undergo multipl..

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Grants

Awarded by Australian Research Council Centre of Excellence in Convergent Bio-Nano Science and Technology


Awarded by Australian Research Council


Awarded by National Health and Medical Research Council


Funding Acknowledgements

This research was conducted and funded by the Australian Research Council (ARC) Centre of Excellence in Convergent Bio-Nano Science and Technology (project number CE 140100036) and an ARC Discovery Project (DP170103331). F.C. acknowledges the award of a National Health and Medical Research Council Senior Principal Research Fellowship (GNT1135806). This work was performed in part at the Materials Characterization and Fabrication Platform and the Bio21 Advanced Microscopy Facility at The University of Melbourne and the Victorian Node of theAustralian National Fabrication Facility. We thank Dr. Shuaijun Pan, Yutian Ma, Jingqu Chen, Yingjie Hu, and Jiaying Song for assistance with experiments, Wenjie Zhang for providing the green fluorescent proteins, Dr. Christina Cortez-Jugo, Dr. Yi Ju, and Dr. Md. Arifur Rahim for helpful discussions, and Dr. Yee-Foong Mok (Macromolecular Interactions Facility Platform, Bio21 Institute, The University of Melbourne) for assistance with the circular dichroism analysis.