Journal article
Ocular Phenotype of Relaxin Gene Knockout (Rln-/-) Mice
U Hampel, HR Chinnery, F Garreis, F Paulsen, R de Iongh, BV Bui, C Nguyen, L Parry, C Huei Leo
Current Eye Research | TAYLOR & FRANCIS INC | Published : 2020
Abstract
Purpose: To test if relaxin deficiency affects ocular structure and function we investigated expression of relaxin (Rln) and RXFP receptors (Rxfp1, Rxfp2), and compared ocular phenotypes in relaxin gene knockout (Rln−/−) and wild type (Rln+/+) mice. Materials and Methods: Rln, Rxfp1 and Rxfp2 mRNA expression was detected in ocular tissues of Rln+/+ mice using RT-PCR. The eyes of 11 Rln−/− and 5 Rln+/+ male mice were investigated. Corneal and retinal thickness was assessed using optical coherence tomography. Intraocular pressure was measured using a rebound tonometer. Retinal, choroidal and sclera morphology and thickness were evaluated histologically. Eyes were collected and fixed for immuno..
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Awarded by Boehringer Ingelheim
Funding Acknowledgements
This work was supported by Deutsche Forschungsgemeinschaft (DFG) [HA 6344/2-1] and Boehringer Ingelheim travel grants. This project was partially funded by Novartis Pharma, as a condition of the Australian Research Council Linkage Grant that funded this research.