Analysis of genomic-length HBV sequences to determine genotype and subgenotype reference sequences
Anna L McNaughton, Peter A Revill, Margaret Littlejohn, Philippa C Matthews, M Azim Ansari
Journal of General Virology | MICROBIOLOGY SOC | Published : 2020
Hepatitis B virus (HBV) is a diverse, partially double-stranded DNA virus, with 9 genotypes (A-I), and a putative 10th genotype (J), characterized thus far. Given the broadening interest in HBV sequencing, there is an increasing requirement for a consistent, unified approach to HBV genotype and subgenotype classification. We set out to generate an updated resource of reference sequences using the diversity of all genomic-length HBV sequences available in public databases. We collated and aligned genomic-length HBV sequences from public databases and used maximum-likelihood phylogenetic analysis to identify genotype clusters. Within each genotype, we examined the phylogenetic support for curr..View full abstract
Awarded by Wellcome Trust
P. M. is funded by the Wellcome Trust (grant number 110110).