Membrane association of a model CD4( ) T-cell vaccine antigen confers enhanced yet incomplete protection against murid herpesvirus-4 infection
Joseph Yunis, Alec J Redwood, Gabrielle T Belz, Philip G Stevenson
Immunology & Cell Biology | WILEY | Published : 2020
Vaccination against γ-herpesviruses has proved difficult. CD4+ T cells are essential to contain infection, but how best to prime them and whether this can reduce viral loads remain unclear. To address these questions, we used ovalbumin (OVA) as a model antigen, delivering it with murine cytomegalovirus (MCMV) to protect mice against OVA-expressing murine herpesvirus-4 (MuHV-4). Membrane-associated OVA (mOVA) was more effective than soluble OVA, both to prime CD4+ T cells and as an effector target. It was also a better target than an OVA epitope limited to infected cells, suggesting that protective CD4+ T cells recognize infected cell debris rather than infected cells themselves. While MCMV-m..View full abstract
Awarded by National Health and Medical Research Council
Awarded by Australian Research Council
The work was supported by grants from the National Health and Medical Research Council (project grants 1079180, 1122070, 1140169), the Australian Research Council (grant DP190101851) and by Queensland Health. DO.11.10 cells were kindly provided by Professor P Marrack (University of Colorado).