Journal article

Role of Plasmodium falciparum Protein GEXPO7 in Maurer's Cleft Morphology, Knob Architecture, and P. falciparum EMP1 Trafficking

Emma McHugh, Olivia MS Carmo, Adam Blanch, Oliver Looker, Boyin Liu, Snigdha Tiash, Dean Andrew, Steven Batinovic, Andy JY Low, Hyun-Jung Cho, Paul McMillan, Leann Tilley, Matthew WA Dixon

MBIO | AMER SOC MICROBIOLOGY | Published : 2020

Abstract

The malaria parasite Plasmodium falciparum traffics the virulence protein P. falciparum erythrocyte membrane protein 1 (PfEMP1) to the surface of infected red blood cells (RBCs) via membranous organelles, known as the Maurer's clefts. We developed a method for efficient enrichment of Maurer's clefts and profiled the protein composition of this trafficking organelle. We identified 13 previously uncharacterized or poorly characterized Maurer's cleft proteins. We generated transfectants expressing green fluorescent protein (GFP) fusions of 7 proteins and confirmed their Maurer's cleft location. Using co-immunoprecipitation and mass spectrometry, we generated an interaction map of proteins at th..

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Grants

Awarded by National Health and Medical Research Council


Awarded by National Health and Medical Research Council Training fellowship


Awarded by Australian Research Council


Funding Acknowledgements

L.T. is a Georgina Sweet, Australian Research Council Laureate Fellow (LE150100011) (http://www.arc.gov.au).M.W.A.D.and L.T. thank the Australian Research Council (DP110100624) (http://www.arc.gov.au) and the National Health and Medical Research Council (1098992) (https://www.nhmrc.gov.au) for funding this work. M.W.A.D. was supported by a National Health and Medical Research Council Training fellowship (602541).