Characterization of the molecular basis of group II intron RNA recognition by CRS1-CRM domains.
Ido Keren, Liron Klipcan, Ayenachew Bezawork-Geleta, Max Kolton, Felix Shaya, Oren Ostersetzer-Biran
Journal of Biological Chemistry | American Society for Biochemistry & Molecular Biology (ASBMB) | Published : 2008
CRM (chloroplast RNA splicing and ribosome maturation) is a recently recognized RNA-binding domain of ancient origin that has been retained in eukaryotic genomes only within the plant lineage. Whereas in bacteria CRM domains exist as single domain proteins involved in ribosome maturation, in plants they are found in a family of proteins that contain between one and four repeats. Several members of this family with multiple CRM domains have been shown to be required for the splicing of specific plastidic group II introns. Detailed biochemical analysis of one of these factors in maize, CRS1, demonstrated its high affinity and specific binding to the single group II intron whose splicing it fac..View full abstract