Journal article
AtnMat2, a nuclear-encoded maturase required for splicing of group-II introns in Arabidopsis mitochondria
I Keren, A Bezawork-Geleta, M Kolton, I Maayan, E Belausov, M Levy, A Mett, D Gidoni, F Shaya, O Ostersetzer-Biran
RNA | COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT | Published : 2009
DOI: 10.1261/rna.1776409
Abstract
Mitochondria (mt) in plants house about 20 group-II introns, which lie within protein-coding genes required in both organellar genome expression and respiration activities. While in nonplant systems the splicing of group-II introns is mediated by proteins encoded within the introns themselves (known as "maturases"), only a single maturase ORF (matR) has retained in the mitochondrial genomes in plants; however, its putative role(s) in the splicing of organellar introns is yet to be established. Clues to other proteins are scarce, but these are likely encoded within the nucleus as there are no obvious candidates among the remaining ORFs within the mtDNA. Intriguingly, higher plants genomes con..
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Awarded by U.S-Israel Binational Agricultural Research and Development Fund
Awarded by U.S-Israel Binational Science Foundation
Awarded by Israeli Science Foundation
Funding Acknowledgements
We thank Professor Ian Small (University of Western Australia), Professor Valerian V. Dolja (Oregon State University), and Dr. Yoram Eyal (Volcani Center, Agricultural Research Organization) for allowing us to use their GFP-CysRS, Cherry-PTS1, and GFP-RbcS markers, respectively; Dr. Yuval Eshed (Weizmann Institute) for providing us with the binary pML-BART vector system; and Professor J.M Grienenberger, Dr. Philippe Giege (CNRS, Strasbourg), and Professor Zach Adam (the Hebrew University) for providing us with the antibodies to NAD9, ccmFc, and PsbO, respectively. We also wish to thank Mrs. Leah Naveh (the Hebrew University) for help with respiration analysis and Dr. Ron Ophir (Volcani center, Agricultural Research Organization) for his assistance with phylogenetic analysis. This work was supported by grants from the U.S-Israel Binational Agricultural Research and Development Fund (BARD IS-374-05), the U.S-Israel Binational Science Foundation (BSF 2007200), and in part by the Israeli Science Foundation (ISF 1176/07).