Journal article
Cationic Nanoliposomes Meet mRNA: Efficient Delivery of Modified mRNA Using Hemocompatible and Stable Vectors for Therapeutic Applications
T Michel, D Luft, MK Abraham, S Reinhardt, ML Salinas Medina, J Kurz, M Schaller, M Avci-Adali, C Schlensak, K Peter, HP Wendel, X Wang, S Krajewski
Molecular Therapy Nucleic Acids | CELL PRESS | Published : 2017
Abstract
Synthetically modified mRNA is a unique bioactive agent, ideal for use in therapeutic applications, such as cancer vaccination or treatment of single-gene disorders. In order to facilitate mRNA transfections for future therapeutic applications, there is a need for the delivery system to achieve optimal transfection efficacy, perform with durable stability, and provide drug safety. The objective of our study was to comprehensively analyze the use of 3β-[N-(N',N'-dimethylaminoethane) carbamoyl](DC-Cholesterol)/dioleoylphosphatidylethanolamine (DOPE) liposomes as a potential transfection agent for modified mRNAs. Our cationic liposomes facilitated a high degree of mRNA encapsulation and success..
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Funding Acknowledgements
The staining and imaging of liposomes with TEM were kindly provided by the work group of Prof. Martin Schaller. M.-K.A. is supported by the German research association (Deutsche Forschungsgemeinschaft). K.P. was supported by a National Health and Medical Research Council Principal Research Fellowship. X.W. is supported by a National Heart Foundation Postdoctoral Fellowship with the Paul Korner Innovation Award. S.K. was supported by the Margarete von Wrangell Habilitation Programme for Women of the Ministry of Science and Arts Baden-Wuerttemberg.